Barcoding fungi: A new era in mycology

October 9, 2015
Burke Museum

Advancements in DNA analysis have opened doors to numerous new areas of research in the biological sciences. In mycology—the study of fungi—DNA sequence data is playing a critical role in answering challenging questions about the identification, distribution, and relationships of fungus species.

Traditional methods of study, including morphology and habitat data, remain important to understanding these organisms, but DNA sequencing allows researchers to more easily identify species, develop in-depth phylogenetic studies of thousands of fungi, study their distribution and migration patterns, and evaluate their presence and function in a variety of terrestrial ecosystems.

Cortinarius parkeri, a common spring species in conifer forest.

Cortinarius parkeri, a common spring species in conifer forest.
Photo: Andrew Parker

Displayed species.

Photo: J. Ammirati

Oregon oak habitat in the Columbia Gorge.

Oregon oak habitat in the Columbia Gorge.
Photo: J. Ammirati

Dr. Joe Ammirati, Burke Museum adjunct curator of mycology, has collaborated extensively for more than three decades with local, regional and international researchers to better understand the hundreds of species that compromise the large mushroom genus Cortinarius, the most common mushrooms found in Western Washington’s north temperate ecosystems.

Currently, Ammirati and colleagues are developing DNA “barcodes”—a unique DNA sequence—that can be used to identify species from mushroom tissue, spores, or environmental samples. These mushrooms with a barcode could be easily identified by non-mushroom experts. The barcodes will be placed in online public databases, allowing full access to the data by everyone, including medical and veterinary staff dealing with mushroom poisonings, who could use the databases to rapidly identify the species of mushroom ingested.

Ammirati and his colleagues are currently conducting a study of the species of Cortinarius that grow in association with Oregon oak trees in the Columbia Gorge, where these trees are part of unique habitats that support a rich array of fungi, plants, animals and other organisms. This is one of the first extensive studies of the fungi associated with Oregon oak in our region. The species of Cortinarius that grow in association with the roots of Oregon oak are almost all new to science and often represent previously unknown evolutionary lineages.

Back to Top